Stable Plastid Transformation in Lettuce (Lactuca sativa L.)
نویسندگان
چکیده
منابع مشابه
Phosphomannose isomerase gene for selection in lettuce (Lactuca sativa L.) transformation.
A positive selection system using phosphomannose isomerase was employed for Agrobacterium tumefaciens mediated transformation of lettuce (Lactuca sativa L. var. 'Achát'). It was shown that the mannose-based selection system works very well with the lettuce genotype used, reaching up to 25% transformation efficiency on the medium with 20 g/L mannose and 20 g/L sucrose. The best transformation ef...
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Lactuca sativa is widely used as a leafy vegetable belonging to the Asteraceae family. Cotyledonary leaves of in vitro lettuce seedlings were transformed by plasmids containing the GCHI gene. Also the effects of number of days of pre-cultivation and co-cultivation of explants were examined on the transformation frequency. After co-cultivation, the explants were transferred onto the regeneration...
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Lettuce (Lactuca sativa L.) is one of the most important leafy vegetable that is consumed during its vegetative growth. The transition from vegetative to reproductive growth is induced by high temperature, which has significant economic effect on lettuce production. However, the progression of floral transition and the molecular regulation of bolting are largely unknown. Here we morphologically...
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As part of our investigation of disease resistance in lettuce, we generated mutants that have lost resistance to Bremia lactucae, the casual fungus of downy mildew. Using a rapid and reliable screen, we identified 16 distinct mutants of Latuca sativa that have lost activity of one of four different downy mildew resistance genes (Dm). In all mutants, only a single Dm specificity was affected. Ge...
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The antibiotic patiilin (I) has been reported to inhibit seed germination becauise of its toxicity (7). We considered that this butenolide (I) might induce light sensitive dormancy in seeds of lettuce because of its structural similarity to coumarin (II) and ramulosin (III), (see fig 1), which have been demonstrated to possess this biological activity (2,5,6). Possible activitv was tested in th...
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ژورنال
عنوان ژورنال: Plant Molecular Biology
سال: 2005
ISSN: 0167-4412,1573-5028
DOI: 10.1007/s11103-005-7704-8